Class I β-tubulin mutations in Epo-resistant cell lines.
Total RNA was prepared from Epo-resistant cell lines and the parental drug-sensitive cell lines. RNA was reverse transcribed to cDNA. The class I β-tubulin gene was amplified by PCR and sequenced with four pairs of primers. A mutation is noted after comparison to both the published sequence and the class I β-tubulin in the drug-sensitive parental cell line.
Cell line | Amino acid | Codon |
---|---|---|
A549.EpoB40 | B292Gln → Glu | CAG → GAG |
HeLa.EpoA9 | B173Pro → Ala | CCC → GCC |
HeLa.EpoB1.8 | B422Tyr → Tyr/Cys | TAT → TAT/TGT |