Table 4

Class I β-tubulin mutations in Epo-resistant cell lines.

Total RNA was prepared from Epo-resistant cell lines and the parental drug-sensitive cell lines. RNA was reverse transcribed to cDNA. The class I β-tubulin gene was amplified by PCR and sequenced with four pairs of primers. A mutation is noted after comparison to both the published sequence and the class I β-tubulin in the drug-sensitive parental cell line.

Cell lineAmino acidCodon
A549.EpoB40B292Gln → GluCAG → GAG
HeLa.EpoA9B173Pro → AlaCCC → GCC
HeLa.EpoB1.8B422Tyr → Tyr/CysTAT → TAT/TGT