Table 1

Primer and probe sequences and optimal concentrations

TargetSequenceOptimal conc. (nm)
TP (human)
 FwdGGAGAAGGGTGACCGACTCA100
 ProbeFAM-CGCTGAGATCAATCGGCCCGACTAT-TAMRA200
 RevTGCCCAGACTCGGCAAAG200
TP (mouse)
 FwdCGGCCAGAGTGCAAAGCT200
 ProbeFAM-CAGCATACAGGATCCCATCAGCAGGAA-TAMRA200
 RevTCCACAGTGGCTGTCACATCTC300
TNF-α
 FwdGGAGAAGGGTGACCGACTCA300
 ProbeFAM-CGCTGAGATCAATCGGCCCGACTAT-TAMRA250
 RevTGCCCAGACTCGGCAAAG200
S9
 FwdATCCGCCAGCGCCATA100
 ProbeFAM-AGCAGGTGGTGGTGAACATCCCGTCCTT-TAMRA300
 RevTCAATGTGCTTCTGGGAATCC100
DPD
 FwdCCAAAGGCAGTAAAGCAGGAA300
 ProbeFAM-TGCGCCTGTCACTCTCCATTGCC-TAMRA25
 RevTCACGACTCCCCGTATCGA100