Table 2

Inhibition of NO production suppressed 4-HPR-mediated growth inhibition in breast cancer cells

4-HPR (1 μm)l-NMMAam)Cell countb (× 104)Nitritecm/106 cells)
T47D cells
 −68.5 ± 1.813.7 ± 0.7
 +21.5 ± 1.363.3 ± 3.8
 −  167 ± 2.812.3 ± 0.5
 − 1060 ± 2.212.6 ± 0.9
 −10074 ± 2.612.3 ± 0.6
 +  123 ± 1.843.3 ± 2.7
 + 1050 ± 2.619.9 ± 1.0
 +10072 ± 3.013.9 ± 0.6
MCF-7 cells
 −264 ± 5.66.5 ± 0.6
 +27.5 ± 1.365.9 ± 2.1
 −  1263 ± 3.66.7 ± 0.5
 − 10264 ± 8.27.0 ± 0.4
 −100260 ± 4.46.6 ± 1.1
 +  130 ± 2.063.4 ± 3.2
 + 10112 ± 3.628.2 ± 1.6
 +100228 ± 5.38.1 ± 0.7
  • a NOS competitive inhibitor.

  • b Total live cell counts were determined by trypan blue exclusion. Values are means ± SD of experiments performed in triplicate.

  • c Total NO production was determined by measuring its stable end product nitrite. Nitrite values were normalized to cell number. Values are means ± SD of experiments performed in triplicate.