Table 1

4-HPR induced growth inhibition and NO production in ER+ and ER− breast cancer cells

4-HPR (μm)ER-positive
ER-negative
MCF-7T47DMDA-MB-453SKBr-3
A. Growth inhibition, total cell count (× 104)a
0264.0 ± 2.9860.5 ± 2.2464.5 ± 2.6455.0 ± 2.37
128.0 ± 1.4118.0 ± 0.5040.0 ± 1.7323.0 ± 0.87
2.5
7.3 ± 0.289
6.5 ± 0.50
20.0 ± 0.43
12.5 ± 0.43
B. NO production, μm nitrite/1 × 106 cellsb
06.5 ± 0.6811.1 ± 0.1619.6 ± 0.3322.6 ± 2.41
164.4 ± 2.4080.9 ± 1.6131.9 ± 1.4144.2 ± 0.41
2.5230.7 ± 7.6227.4 ± 1.2963.6 ± 6.3676.3 ± 1.55
  • a Total live cell counts were determined by trypan blue exclusion. The values reported are the means (±SD) of experiments performed in triplicate.

  • b Total NO production was determined by measuring its stable end product nitrite. Nitrite values were normalized to cell number. Values reported are the means (±SD) of experiments performed in triplicate.