PT  - JOURNAL ARTICLE
AU  - Ban, Jung Ok
AU  - Oh, Ju Hoon
AU  - Hwang, Bang Yeon
AU  - Moon, Dong Cheul
AU  - Jeong, Heon-Sang
AU  - Lee, Seram
AU  - Kim, Soyoun
AU  - Lee, Hyosung
AU  - Kim, Kyung-Bo
AU  - Han, Sang Bae
AU  - Hong, Jin Tae
TI  - Inflexinol inhibits colon cancer cell growth through inhibition of nuclear factor-κB activity via direct interaction with p50
AID  - 10.1158/1535-7163.MCT-08-0694
DP  - 2009 Jun 01
TA  - Molecular Cancer Therapeutics
PG  - 1613--1624
VI  - 8
IP  - 6
4099  - http://mct.aacrjournals.org/content/8/6/1613.short
4100  - http://mct.aacrjournals.org/content/8/6/1613.full
SO  - Mol Cancer Ther2009 Jun 01; 8
AB  - Kaurane diterpene compounds have been known to be cytotoxic against several cancer cells through inhibition of nuclear factor-κB (NF-κB) activity. Here, we showed that inflexinol, a novel kaurane diterpene compound, inhibited the activity of NF-κB and its target gene expression as well as cancer cell growth through induction of apoptotic cell death in vitro and in vivo. These inhibitory effects on NF-κB activity and on cancer cell growth were suppressed by the reducing agents DTT and glutathione and were abrogated in the cells transfected with mutant p50 (C62S). Sol-gel biochip and surface plasmon resonance analysis showed that inflexinol binds to the p50 subunit of NF-κB. These results suggest that inflexinol inhibits colon cancer cell growth via induction of apoptotic cell death through inactivation of NF-κB by a direct modification of cysteine residue in the p50 subunit of NF-κB. [Mol Cancer Ther 2009;8(6):1613–24]