Article Figures & Data
Figures
- Figure 1.
Chemical structures of IMP-288 and IMP-449. Both are Tyr-d-Lys-d-Glu-d-Lys tetrapeptides in which both lysine residues are substituted with a HSG moiety via their ε-amino group. IMP-288 is conjugated with DOTA, and IMP-449 is conjugated with NOTA.
- Figure 2.
Biodistribution of 111In-IMP-288 1 h after i.v. injection, following pretargeting with escalating doses of TF2 in BALB/c nude mice with a s.c. CEA-expressing LS174T tumor. Two peptide doses were tested: 0.01 nmol 111In-IMP-288 (A) and 0.10 nmol 111In-IMP-288 (B). Values are given as means ± SD (n = 5).
- Figure 3.
A, biodistribution of 6.0 nmol 125I-TF2 (0.37 MBq) and 0.25 nmol 68Ga-IMP-288 (5 MBq) 1 h after i.v. injection of 68Ga-IMP-288 in BALB/c nude mice with a s.c. LS174T and SK-RC 52 tumor. Values are given as means ± SD (n = 5). B, three-dimensional volume rendering of PET/CT image of a BALB/c nude mouse with a s.c. LS174T CEA-expressing tumor in the right flank (arrow) and a s.c. SK-RC 52 tumor, a non–CEA-producing tumor, in the left flank (arrowhead), which received 6.0 nmol TF2 and 5 MBq 68Ga-IMP-288 (0.25 nmol) i.v. with a 16-h interval, imaged 1 h after 68Ga-IMP-288 injection.
- Figure 4.
PET/CT images of a BALB/c nude mouse with a s.c. LS174T tumor (0.1 g) on the right hind leg (arrow) and an inflammation in the left thigh muscle (arrowhead), which received 5 MBq 18F-FDG and, 1 d later, 6.0 nmol TF2 and 5 MBq 68Ga-IMP-288 (0.25 nmol) with a 16-h interval. The animal was imaged 1 h after 18F-FDG and 68Ga-IMP-288 injections. The panel shows the three-dimensional volume rendering of the pretargeted immuno-PET scan (A) and the FDG-PET scan (B), and the transverse sections of the tumor region of the pretargeted immuno-PET scan (C) and the FDG-PET scan (D).
- Figure 5.
Biodistribution of 5 MBq FDG and 5 MBq 68Ga-IMP-288 (0.25 nmol) 1 h after injection, following pretargeting with 6.0 nmol TF2 in BALB/c nude mice with a s.c. CEA-expressing LS174T tumor. Values are given as means ± SD (n = 5).
- Figure 6.
Biodistribution of 0.25 nmol 18F-IMP-449 (5 MBq) 1 h after injection, following pretargeting with 6.0 nmol TF2 16 h earlier, biodistribution of 18F-IMP-449 without pretargeting, or biodistribution of Al[18F]2+ in BALB/c nude mice with a s.c. CEA-expressing LS174T tumor. Values are given as means ± SD.
- Figure 7.
Static PET/CT imaging study of a BALB/c nude mouse with a s.c. LS174T tumor (0.1 g) on the right side, which received 6.0 nmol TF2 and 0.25 nmol 18F-IMP-449 (5 MBq) i.v. with a 16-h interval. The animal was imaged 1 h after injection of 18F-IMP-449. The panel shows the three-dimensional volume rendering (posterior view; A) and cross-sections at the tumor region [coronal (B), sagittal (C), and transversal (D)].
Volume 9, Issue 4
