Immunofluorescent image of untreated A549 lung adenocarcinoma cells. A549 cells seeded on collagen coated coverslips were stained for focal adhesions (antivinculin antibody; green) and F-Actin stress fibers (rhodamine conjugated phalloidin; red). Nuclei were visualized by DAPI staining (blue). It was found that Nutlin 3, a small molecule inhibitor of MDM2, decreases F-actin stress fibers, focal adhesions and invasion in p53wt human cancer cells. For details, see article by Moran and Maki on page 895.