Article Figures & Data
Figures
- Figure 1.
Levels of mature miR-21 expression in human pancreatic cell lines and tissues. A, levels of miR21 expression relative to HPDE as assessed by qRT-PCR and normalized to the level of a housekeeping gene, U6 mRNA in each sample. B, levels of miR-21 expression in 25 cancer tissues and 25 nonmalignant tissues as assessed by qRT-PCR and normalized to U6 mRNA (*, P < 0.001, Mann-Whitney). Mean ± SD of triplicate measurements.
- Figure 2.
Effects of miR-21 on cell proliferation of pancreatic cancer cells. Pancreatic cancer cell lines were transfected with the miR-21 precursor or inhibitor, and their cell proliferation rates were assessed by the PI assay. A, increased proliferation of PANC-1 cells was seen with miR-21 precursor transfection (1.8-fold) compared with the controls at 120 h after seeding (*, P < 0.001, Student's t test). B, decreased proliferation of CFPAC-1, AsPC-1, and PANC-1 cells was seen with miR-21 inhibitor transfection compared with the control at 120 h after seeding (*, P < 0.001; **, P = 0.019; ***, P = 0.003, respectively, Student's t test). Mean ± SD of triplicate measurements.
- Figure 3.
Effects of miR-21 on invasion of pancreatic cancer cells. PANC-1 and CFPAC-1 cells were transfected with the miR-21 precursor or inhibitor, compared with the cells transfected each control sequence, and their cell invasion activities were assessed after 72 h. A, increased Matrigel invasion with PANC-1 cells was seen with miR-21 transfection (2.2-fold compared with control cells; *, P = 0.002, Student's t test). B, decreased Matrigel invasion with CFPAC-1 cells was seen with miR-21 inhibitor transfection (0.3-fold compared with control cells; *, P < 0.001, Student's t test). Mean ± SD of triplicate measurements.
- Figure 4.
Effects of miR-21 on MMP-2, MMP-9, and VEGF mRNA expression. A, MMP-2 and MMP-9 mRNA were assessed by qRT-PCR. miR-21 modulates positively the mRNA expression of MMP-2 and MMP-9 (*, P = 0.002; **, P = 0.010, Student's t test). B, VEGF mRNA was also assessed by qRT-PCR. miR-21 positively modulates VEGF mRNA expression (*, P = 0.042; **, P = 0.005, Student's t test). Mean ± SD of triplicate measurements.
- Figure 5.
Effects of miR-21 on pancreatic cancer cell chemoresistance. PANC-1 and SUIT-2 cells were transfected with the miR-21 precursor or inhibitor, compared with the cells transfected with the control sequences, and their chemosensitivities were assessed using the PI assay 72 h after treatment with 1 to 1,000 nmol/L gemcitabine. A, survival rate of PANC-1 cells transfected with the miR-21 precursor was higher than that of the cells transfected with the negative control precursor after treatment with 10 to 100 nmol/L gemcitabine (*, P < 0.01, Student's t test). B, apoptosis assay was done by flow cytometry using Annexin V and PI after 24 h the treatment of 1 μmol/L gemcitabine. PANC-1 cells tranfected with miR-21 precursor reduced the number of apoptotic cells compared with the controls. The rate of Annexin V-positive cells was 20.3% in PANC-1 cells transfected with miR-21 precursor and 41.8% in the control cells (*, P = 0.008, Student's t test). C, survival rate of SUIT-2 cells transfected with the miR-21 inhibitor was lower than that of the cells transfected with the negative control inhibitor after treatment with 2 to 20 nmol/L gemcitabine (*, P = 0.005; **, P = 0.017; ***, P < 0.001; ****, P = 0.0037, Student's t test). Mean ± SD of triplicate measurements.
Additional Files
Supplementary Materials, Moriyama et al
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Volume 8, Issue 5
