Chimeric antigen receptor T (CAR T) cells represent a new paradigm in cancer therapy. However, viral-based production of these CAR T cells is laborious and comes at a high cost. Therefore, Cai and colleagues sought to utilize minicircle DNA (mcDNA) to produce CAR T cells without the need for viruses. In the cover image, the authors demonstrate strong expression of PSCA on a prostate xenograft. CAR T cells derived using mcDNA targeted at this PSCA infiltrated the tumor and increased cytokine secretion. Read the article on page 178.