Article Figures & Data
Figures
- Figure 1.
Blood pharmacokinetic curves of 125I-labeled (A) and 111In-labeled (B) antibodies at various doses through 7 days. Solid data symbols correspond to control mice, whereas open symbols indicate mice treated with anti-VEGF at 24 hours before tracer administration.
- Figure 2.
Tumor pharmacokinetic curves with and without anti-VEGF (B20.4.1) pretreatment: trastuzumab at doses of 0.1 mg/kg (A), 1.4 mg/kg (B), 17 mg/kg (C), and a control IgG at a dose of 18 mg/kg (D).
- Figure 3.
Tissue distribution of 111In-labeled (A and B) and 125I-labeled (C and D) antibodies at 120-hour postinjection. Data in B and D are derived from mice receiving anti-VEGF at 24 hours before tracer injection.
- Figure 4.
Absolute difference in AUC (%ID/g × d), relative to control, due to the effect of a 24-hour pretreatment of the anti-VEGF antibody, B20-4.1.
- Figure 5.
SPECT-CT (A–D) and autoradiograms with their respective pseudocolor heat map (E and F) imaging data acquired in mice following intravenous administration of 111In-trastuzumab. For SPECT-CT images, 3-dimensional volume renderings from a coronal perspective (top), coronal tomographic slices (middle), and axial tomographic slices (bottom) are displayed. Mice received 111In-trastuzumab tracer only (A and C) or were coinjected with a dose of 14 to 17 mg/kg (B, D–F). Only mice in C, D, and F received a single intravenous dose of anti-VEGF (10 mg/kg) at 24 hours before tracer injection.
- Figure 6.
Immunohistochemical staining of KPL-4 tumor sections for the MECA-32 vascular endothelium marker (brown; A, B), HER2 receptors (pink; C, D), and bound trastuzumab (blue; E, F). Images in A, C, and E correspond to trastuzumab (17 mg/kg) only treated mice, whereas those in B, D, and F are derived from mice receiving a single anti-VEGF dose (10 mg/kg) 24 hours before trastuzumab. Tumors were harvested at 24 hours after intravenous administration of trastuzumab. Scale bar, 500 μm. Insets display same tumor sections at a larger scale (2.0 mm).
Additional Files
Supplementary Data
Files in this Data Supplement:
- Supplementary Figure 1 - PDF file - 93K
- Supplementary Table 1-2 - PDF file - 87K
Volume 11, Issue 3
