Table 1.

Sequencing of BRCA2 in CAPAN1.B2.S*

Cell lineReference sequence for BRCA2 CAGCAAGTGGAAAATCTGTCCAGGTATCAGATGCTCRISPR-Induced mutationSubcloned colonies sequenced (#)Estimated allele frequency
CAPAN-1CAGCAAG-GGAAAATCTGTCCAGGTATCAGATGCN/A201.00
CAPAN-1.B2.S*CAGCAAG-GGAAAATCTGTCCAGGTATCAGATGCCAPAN-1(6174delT)200.59
CAGCAAG-GGAAAAT—————CAGGTATCAGATGC5-bp Deletion0.41