Table 3.

M9346A processing in FRα-positive cell lines

IC50, nmol/L (continuous exposure)M9346A processingb
Cell lineIMGN853IMGN853+ M9346AaS-methyl-DM4Receptor density, antibody-binding sites/cell × 1,000% Processedpmol processed/106 cells
KB0.10 ± 0.061.4 ± 0.50.03 ± 0.014,500 ± 64029 ± 12.2 ± 0.4
Igrov0.8 ± 0.310 ± 20.03 ± 0.011,300 ± 79036 ± 10.75 ± 0.40
Jeg-30.9 ± 0.112 ± 50.03 ± 0.02290 ± 13057 ± 10.3 ± 0.1
Skov-37 ± 18 ± 10.06 ± 0.0194 ± 1330 ± 90.05 ± 0.01
Ovcar-33 ± 13.0 ± 0.60.02 ± 0.0148 ± 1424 ± 10.02 ± 0.01
HCC82710 ± 010 ± 00.04 ± 0.0158 ± 1724 ± 70.02 ± 0.01
Ishikawa1.0 ± 0.21.0 ± 0.40.05 ± 0.02100 ± 828 ± 60.05 ± 0.01

NOTE: Data represent the mean ± SD of three independent experiments.

  • aM9346A was used at saturating concentration of 2 μmol/L.

  • bAssessed using 3[H]-M9346A as described in Materials and Methods.