Table 1.

Growth inhibition induced by the Dynoles in a range of human cancer cell lines

Cell line tissueGI50 (μmol/L) after 72-hour exposure
SMA-560HeLaHT29SW480MCF-7A2780H460A431DU145BE2-CSJ-G2
GlialCervicalColonColonBreastOvarianLungSkinProstateNeuronalGlial
Compound
MiTMAB1.6 ± 0.1b0.6 ± 0.1b,d1.0 ± 0.2b1.0 ± 0.3b2.2 ± 0.8a3.9 ± 1.1b0.6 ± 0.0b,d2.7 ± 0.8b1.9 ± 0.7b2.5 ± 0.5a
Dynole 2515 ± 0.4b,c12 ± 0.7b18.7 ± 1.2b,d14.7 ± 0.7b,c24.7 ± 3.0a,c23.5 ± 5.0c29.3 ± 4.7a,c26.0 ± 2.0b,d36.7 ± 0.9b,d19.7 ± 2.8a22.5 ± 3.5a,c
Dynole 266.9 ± 0.17.8 ± 1.616.7 ± 0.3b,d8.4 ± 0.5a13.7 ± 0.3c15.2 ± 0.8d15.2 ± 0.8c14.7 ± 0.3c18.3 ± 0.7b,d14.3 ± 0.3b,c15.0 ± 0.6a,c
Dynole 3310 ± 5.04.0 ± 0.715.0 ± 1.0b,d8.1 ± 0.3b,d15.7 ± 0.3d14.7 ± 1.316.3 ± 0.7d15.3 ± 0.7d24.0 ± 2.9a,d14.0 ± 0.0b,d15.7 ± 0.3a,d
Dynole 34-26.6 ± 0.25.8 ± 0.36.9 ± 0.1c6.5 ± 0.110 ± 1.610 ± 1.614 ± 0.3d13.7 ± 0.3d15 ± 0.0d7 ± 0.1c9.3 ± 1.6
Dynole 3515 ± 0.4b,c13 ± 0.0b16.3 ± 0.3b,d9.1 ± 1.0c13.0 ± 0.615.2 ± 0.815.3 ± 0.7c13.7 ± 0.317.0 ± 0.6a,d13.7 ± 0.3b14.7 ± 0.3a,d

NOTE. Cells were treated with Dynoles for 72 hours followed by MTT assay. The table shows GI50 (half maximal growth inhibitory concentration) values (mean ± SEM) of 5 Dynoles, calculated from the MTT dose–response curves. n = 3. Comparison of each dynamin inhibitor with Dynole 34-2 within an individual cell line:

  • aP < 0.05;

  • bP < 0.01. Comparison of an individual dynamin inhibitor in different cell lines with HeLa cells:

  • cP < 0.05.

  • dP < 0.01.