Table 3.

BN82685 inhibits the growth of human cell lines resistant to cytotoxic drugs

Human cell linesIC50 (nmol/L)
BN82685DNRADRMTZVP-16
Acute promyelocytic leukemia*HL606073288NDND
Acute promyelocytic leukemia resistant to daunorubicinHL60-DNR(pgp)6101,1003,100NDND
Acute promyelocytic leukemia resistant to AdriamycinHL60-ADR(MRP)6436942,430NDND
Acute promyelocytic leukemia*HL60480NDND<100ND
Acute promyelocytic leukemia resistant to mitoxantroneHL60/MX2480NDND600ND
Acute lymphoblastic leukemiaCCRF-CEM300NDNDND160
Acute lymphoblastic leukemia resistant to VP-16CCRF-CEM/C2290NDNDND1,810
  • NOTE: Growth inhibition of the indicated cell lines was assayed using the WST1 cell proliferation assay. The IC50 (in millimolar) values reported were calculated from two independent experiments with four or eight determinations per concentration tested (see Materials and Methods for details).

  • Abbreviations: DNR, daunorubicin; ADR, Adriamycin; MTZ, mitoxantrone; VP-16, etoposide; MRP, multidrug resistance protein; pgp, P-glycoprotein; ND, not determined.

  • * The results presented for the acute promyelocytic leukemia cell line HL60 were obtained from two sets of independent experiments.