Table 1.

BN82685 inhibits purified recombinant members of CDC25 family

BN82685 hydrochloride salt
BN82685 benzoate salt
IC50 (nmol/L)SEIC50 (nmol/L)SE
CDC25C171220137
CDC25C-cat1797011711
CDC25A251801095
CDC25B2250816021
CDC25B32604124956
  • NOTE: Enzyme inhibition was assayed on recombinant maltose binding protein-CDC25C, maltose binding protein-CDC25C catalytic domain (CDC25C-cat), maltose binding protein-CDC25B2, and maltose binding protein-CDC25B3 using 3-O-methylfluorescein phosphate as a substrate. The IC50 (in nanomolar) values were calculated from at least three independent experiments with one determination per concentration tested.