Table 1.

MMP-2 inhibitory effects of flavonoids with their cytotoxicy in human colorectal COLO 205 cells

CompoundCytotoxicity (%)*MMP-2 inhibition (%)
Flavone71 ± 0.23
3-OH flavone47 ± 0.1
5-OH flavone23 ± 0.11
6-OH flavone38 ± 0.12
7-OH flavone31 ± 0.09
2′-CH3O flavone47 ± 0.06
3-CH3O flavone33 ± 0.00111
5-CH3O flavone30 ± 0.012
6-CH3O flavone25 ± 0.12
Kaempferol35 ± 0.1347
Quercetin59 ± 0.12
Quercitrin9 ± 0.18
Myricetin23 ± 0.1190
Myricitrin9 ± 0.08
Baicalein47 ± 0.12
Baicalin20 ± 0.15
Morin16 ± 0.1
Wogonin71 ± 0.1
Luteolin46 ± 0.1113
Rutin13 ± 0.11
Flavanone24 ± 0.1120
2′-OH flavanone69 ± 0.13
4′-OH flavanone54 ± 0.09
6-OH flavanone55 ± 0.1411
7-OH flavanone21 ± 0.13
4′-CH3O flavanone27 ± 0.1210
5-CH3O flavanone25 ± 0.1115
6-CH3O flavanone23 ± 0.1611
7-CH3O flavanone18 ± 0.2412
Taxifoin19 ± 0.21
Naringenin21 ± 0.17
Naringin20 ± 0.13
Hesperidin23 ± 0.07
Hesperetin21 ± 0.01
(±)Catechin15 ± 0.1
(+)Catechin17 ± 0.1111
(−)catechin45 ± 0.07
  • * Human colorectal COLO 205 cells were treated with indicated compounds (200 μmol/L) for 24 hours and the cell viability was analyzed by MTT assay. The cytotoxicity was measured by the equation [(absorbance of control group) − (absorbance of compound-treated group)]/(absorbance of control group) × 100%.

  • Human colorectal COLO 205 cells were treated with indicated compounds (200 μmol/L) for 24 hours. The conditioned media were collected and normalized by cell numbers before gelatin zymography analysis. The density of each band was quantified by densitometry analysis. The inhibition percentage was measured by the equation [(density of control group) − (density of compound-treated group)]/(density of control group) × 100%.