Table 4.

Effect of AITC and BITC on HL60/S cell cycle progression

Note: Cells were grown in 10-cm plates for 24 h and then exposed to 10 μm AITC or BITC. The exposure lasted for either 24 h, or for 3 h (followed by growing the cells in ITC-free medium for 21 h). The cells then were harvested and analyzed for cell cycle distribution by flow cytometry. Each value is a mean of three measurements (±SD). Each value labeled with an asterisk is significantly different from the control (t test, P < 0.01).

ITC Exposure
ITC
Cell Cycle Distribution, %
G1SG2-M
3 h ITC + 21 h ITC-freevehicle32.2 ± 2.951.1 ± 1.816.6 ± 2.0
AITC53.6 ± 18.6*33.1 ± 17.513.4 ± 1.3
BITC39.0 ± 13.015.9 ± 9.545.8 ± 20.1*
24 h ITC
vehicle32.0 ± 0.557.9 ± 2.310.1 ± 2.6
AITC57.9 ± 6.0*38.5 ± 8.23.6 ± 2.4
BITC43.8 ± 1.1*37.2 ± 1.218.9 ± 1.4*