Table 1.

Growth inhibition of cultured cancer cells by ITCs

Note: Cells were grown in 96-well plates for 24 h and then exposed to a series of concentrations of an ITC, which lasted for either 72 h, or was terminated at 3 h time point (medium removed and cells washed with culture medium) and then grown in ITC-free medium for 69 h. Cell density in each well then was determined with MTT assay and the IC50 value was calculated based on cell growth curve. Each value is the mean of four determinations (±SD).

Cell Line
IC50m)
3 h ITC + 69 h ITC-Free
72 h ITC
AITCBITCPEITCSFAITCBITCPEITCSF
HL60/S3.3 ± 1.12.0 ± 0.34.0 ± 1.331.5 ± 1.32.5 ± 0.11.8 ± 2.33.6 ± 0.43.4 ± 0.6
8662/S5.3 ± 0.72.1 ± 0.25.3 ± 1.221.3 ± 1.54.0 ± 0.62.2 ± 0.63.1 ± 0.31.9 ± 0.2
MCF-712.3 ± 1.38.4 ± 2.417.5 ± 1.5>506.4 ± 1.14.6 ± 0.511.0 ± 1.26.7 ± 0.5
HepG244.8 ± 3.58.1 ± 0.810.1 ± 0.2>5035.5 ± 5.77.3 ± 0.611.2 ± 2.712.8 ± 1.3
HT-295.9 ± 0.75.4 ± 0.910.0 ± 0.5>504.5 ± 0.25.1 ± 1.49.6 ± 1.110.0 ± 0.1
HaCaT9.5 ± 1.06.0 ± 0.78.4 ± 1.4>505.3 ± 0.44.3 ± 0.76.1 ± 1.06.7 ± 1.0