Table 2

Kinetic parameters of thymidine, (N)-MCT in MC38 and MC38/HSV-tk cell lysatea

MC38MC38/HSV-tk
KM(μm)VMAX(pmol/mg/min)VMAX/KMKM(μm)VMAX(pmol/mg/min)VMAX/KM
Thymidine21.713.40.624.133.88.24
(N)-MCTg25025.278.23.10
  • a Kinetic parameters were calculated by linear regression from Lineweaver-Burk plots as described in “Materials and Methods.”.